addgene rrid addgene 20856 software (Addgene inc)
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addgene rrid addgene 20856 software
Addgene Rrid Addgene 20856 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdisplay+bira+er/pDisplay-BirA-ER+(Plasmid+%2320856)/pm40650904-244-20-21
Average 93 stars, based on 33 article reviews
Addgene Rrid Addgene 20856 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdisplay+bira+er/pDisplay-BirA-ER+(Plasmid+%2320856)/pm40650904-244-20-21
Average 93 stars, based on 33 article reviews
addgene rrid addgene 20856 software - by Bioz Stars,
2026-10
93/100 stars
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Expressing:Article Title: Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelope Article Snippet: Colonies were seeded in wells of a tissue culture-treated 96 well plate (Corning 3595) with 293T selection medium and expanded as necessary. .. 1L of Freestyle 293-F cells (Gibco R79007 ) at a density of 1 million cells/mL in Freestyle Expression Medium (Gibco 12338018) was co-transfected with 350μg of the respective sRT plasmid, 150ug of Furin, and 190μg of Article Title: Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelope. Article Snippet: .. A second round of subcloning was performed on the best first-round subclones via fluorescence-activated cell sorting (FACS, BD FACSMelody), gating on the top 1% of double-positive cells. sRT production 1L of Freestyle 293-F cells (Gibco R79007) at a density of 1 million cells/mL in Freestyle Expression Medium (Gibco 12338018) was co-transfected with 350mg of the respective sRT plasmid, 150mg of Furin, and 190mg of Article Title: Utility of protein-protein binding surfaces composed of anti-parallel alpha-helices and beta-sheets selected by phage display. Article Snippet: .. For production of C-terminally biotinylated proteins, a plasmid encoding the bait-AviTag-8xHis-1D4 fusion and Article Title: Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. Article Snippet: .. Protein preparation for Biolayer interferometry The wild-type RABV-G domain III construct encoding residues 32–59 and 181–259 (GenBank: AAC34683.1) connected by a linker composed of three glycines, with a C-terminal Avitag (sequence: GLNDIFEAQKIEWHE) before C-terminal 63 His tag, and its K226E mutant, were designed and commercially obtained as codon-optimized synthetic cDNA from GeneArt (Thermo Fisher Scientific), and cloned into the pHL-sec mammalian expression vector (Addgene #99845).55 The domain III expression plasmid was transiently co-transfected with Article Title: Utility of protein–protein binding surfaces composed of anti-parallel alpha-helices and beta-sheets selected by phage display Article Snippet: .. For production of C-terminally biotinylated proteins, a plasmid encoding the bait-AviTag-8xHis-1D4 fusion and Plasmid Preparation:Article Title: Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelope Article Snippet: Colonies were seeded in wells of a tissue culture-treated 96 well plate (Corning 3595) with 293T selection medium and expanded as necessary. .. 1L of Freestyle 293-F cells (Gibco R79007 ) at a density of 1 million cells/mL in Freestyle Expression Medium (Gibco 12338018) was co-transfected with 350μg of the respective sRT plasmid, 150ug of Furin, and 190μg of Article Title: Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelope. Article Snippet: .. A second round of subcloning was performed on the best first-round subclones via fluorescence-activated cell sorting (FACS, BD FACSMelody), gating on the top 1% of double-positive cells. sRT production 1L of Freestyle 293-F cells (Gibco R79007) at a density of 1 million cells/mL in Freestyle Expression Medium (Gibco 12338018) was co-transfected with 350mg of the respective sRT plasmid, 150mg of Furin, and 190mg of Article Title: Antibodies capable of binding to the spike protein of coronavirus SARS-CoV-2 Article Snippet: Production of RBDs Plasmids encoding RBDs were transfected into Expi293FTM Cells (ThermoFisher) by PEI, cultured in FreeStyleTM 293 Expression Medium (ThermoFisher) at 30° C. with 8% CO2 for 4 days. .. To express biotinylated RBDs, the RBD-BAP plasmid was co-transfected with Article Title: Utility of protein-protein binding surfaces composed of anti-parallel alpha-helices and beta-sheets selected by phage display. Article Snippet: .. For production of C-terminally biotinylated proteins, a plasmid encoding the bait-AviTag-8xHis-1D4 fusion and Article Title: Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. Article Snippet: .. Protein preparation for Biolayer interferometry The wild-type RABV-G domain III construct encoding residues 32–59 and 181–259 (GenBank: AAC34683.1) connected by a linker composed of three glycines, with a C-terminal Avitag (sequence: GLNDIFEAQKIEWHE) before C-terminal 63 His tag, and its K226E mutant, were designed and commercially obtained as codon-optimized synthetic cDNA from GeneArt (Thermo Fisher Scientific), and cloned into the pHL-sec mammalian expression vector (Addgene #99845).55 The domain III expression plasmid was transiently co-transfected with Article Title: Utility of protein–protein binding surfaces composed of anti-parallel alpha-helices and beta-sheets selected by phage display Article Snippet: .. For production of C-terminally biotinylated proteins, a plasmid encoding the bait-AviTag-8xHis-1D4 fusion and Subcloning:Article Title: Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelope. Article Snippet: .. A second round of subcloning was performed on the best first-round subclones via fluorescence-activated cell sorting (FACS, BD FACSMelody), gating on the top 1% of double-positive cells. sRT production 1L of Freestyle 293-F cells (Gibco R79007) at a density of 1 million cells/mL in Freestyle Expression Medium (Gibco 12338018) was co-transfected with 350mg of the respective sRT plasmid, 150mg of Furin, and 190mg of Fluorescence:Article Title: Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelope. Article Snippet: .. A second round of subcloning was performed on the best first-round subclones via fluorescence-activated cell sorting (FACS, BD FACSMelody), gating on the top 1% of double-positive cells. sRT production 1L of Freestyle 293-F cells (Gibco R79007) at a density of 1 million cells/mL in Freestyle Expression Medium (Gibco 12338018) was co-transfected with 350mg of the respective sRT plasmid, 150mg of Furin, and 190mg of FACS:Article Title: Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelope. Article Snippet: .. A second round of subcloning was performed on the best first-round subclones via fluorescence-activated cell sorting (FACS, BD FACSMelody), gating on the top 1% of double-positive cells. sRT production 1L of Freestyle 293-F cells (Gibco R79007) at a density of 1 million cells/mL in Freestyle Expression Medium (Gibco 12338018) was co-transfected with 350mg of the respective sRT plasmid, 150mg of Furin, and 190mg of Construct:Article Title: Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. Article Snippet: .. Protein preparation for Biolayer interferometry The wild-type RABV-G domain III construct encoding residues 32–59 and 181–259 (GenBank: AAC34683.1) connected by a linker composed of three glycines, with a C-terminal Avitag (sequence: GLNDIFEAQKIEWHE) before C-terminal 63 His tag, and its K226E mutant, were designed and commercially obtained as codon-optimized synthetic cDNA from GeneArt (Thermo Fisher Scientific), and cloned into the pHL-sec mammalian expression vector (Addgene #99845).55 The domain III expression plasmid was transiently co-transfected with Sequencing:Article Title: Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. Article Snippet: .. Protein preparation for Biolayer interferometry The wild-type RABV-G domain III construct encoding residues 32–59 and 181–259 (GenBank: AAC34683.1) connected by a linker composed of three glycines, with a C-terminal Avitag (sequence: GLNDIFEAQKIEWHE) before C-terminal 63 His tag, and its K226E mutant, were designed and commercially obtained as codon-optimized synthetic cDNA from GeneArt (Thermo Fisher Scientific), and cloned into the pHL-sec mammalian expression vector (Addgene #99845).55 The domain III expression plasmid was transiently co-transfected with Mutagenesis:Article Title: Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. Article Snippet: .. Protein preparation for Biolayer interferometry The wild-type RABV-G domain III construct encoding residues 32–59 and 181–259 (GenBank: AAC34683.1) connected by a linker composed of three glycines, with a C-terminal Avitag (sequence: GLNDIFEAQKIEWHE) before C-terminal 63 His tag, and its K226E mutant, were designed and commercially obtained as codon-optimized synthetic cDNA from GeneArt (Thermo Fisher Scientific), and cloned into the pHL-sec mammalian expression vector (Addgene #99845).55 The domain III expression plasmid was transiently co-transfected with Clone Assay:Article Title: Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. Article Snippet: .. Protein preparation for Biolayer interferometry The wild-type RABV-G domain III construct encoding residues 32–59 and 181–259 (GenBank: AAC34683.1) connected by a linker composed of three glycines, with a C-terminal Avitag (sequence: GLNDIFEAQKIEWHE) before C-terminal 63 His tag, and its K226E mutant, were designed and commercially obtained as codon-optimized synthetic cDNA from GeneArt (Thermo Fisher Scientific), and cloned into the pHL-sec mammalian expression vector (Addgene #99845).55 The domain III expression plasmid was transiently co-transfected with In Vivo:Article Title: Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. Article Snippet: .. Protein preparation for Biolayer interferometry The wild-type RABV-G domain III construct encoding residues 32–59 and 181–259 (GenBank: AAC34683.1) connected by a linker composed of three glycines, with a C-terminal Avitag (sequence: GLNDIFEAQKIEWHE) before C-terminal 63 His tag, and its K226E mutant, were designed and commercially obtained as codon-optimized synthetic cDNA from GeneArt (Thermo Fisher Scientific), and cloned into the pHL-sec mammalian expression vector (Addgene #99845).55 The domain III expression plasmid was transiently co-transfected with Polymerase Chain Reaction:Article Title: Activation of goblet-cell stress sensor IRE1β is controlled by the mucin chaperone AGR2. Article Snippet: All indicated mutations were introduced with the QuickChange protocol (Agilent), and sequences were verified by Sanger sequencing. .. BirA was PCR-amplified from |
